Folate determination in human milk.
نویسندگان
چکیده
extraction of folates in the absence of a reducing agent, such as ascorbate, is known to be unsuitable for biological samples that contain principaLly reduced tbrms of tblate. When the method by Allfrey ct al. (2) is used for biological samples, the values are generally lower than those obtained by other methods, such as microbiological assay {4). The use of a reducing agent during the extraction procedure to protect reduced folates is essen-
منابع مشابه
The folate in human milk1’2
In previous studies of the folate content of human milk, samples were prepared for assay by a method that resulted in a turbid solution that was then assayed by a turbidimetric microbiological method. We have used an improved microbiological assay in which the milks were treated with rennin to precipitate casein and heated in a buffered ascorbate to coagulate lactalbumin and lactoglobulin. Milk...
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Serum folate determination by this method requires incubation of 0.1 ml of serum (or standards) with nondialyzed milk and then with tritium-labeled folate. A serum blank corrects for nonspecific binders present in the serum. Unbound folate is removed with hemoglobin-coated charcoal. The standard deviation of the results is ±G.2 pig/liter, and more than 90% of folate added to the serum is accoun...
متن کاملEffect of cow milk on food folate bioavailability in young women.
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متن کاملHuman milk folate and folate status in lactating mothers and their infants.
Plasma and red blood cell folate levels of healthy, well-nourished lactating mothers were measured. Folate levels in their breast-fed infants were significantly higher than in the mothers. No abnormal hematological findings were observed in either mothers or infants. Folate levels in breast milk and in the infants' plasma were significantly correlated. The mean breast-milk folate level was 141....
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ورودعنوان ژورنال:
- Journal of nutritional science and vitaminology
دوره 52 2 شماره
صفحات -
تاریخ انتشار 2006